pixelatorR provides the infrastructure to process, analyze and visualize MPX and Proximity Network Assay (PNA) data for R users.
Installation
pixelatorR can be installed from GitHub by running the following code from R:
install.packages("remotes")
remotes::install_github("PixelgenTechnologies/pixelatorR")
Installation from source
You can also install pixelatorR from source by cloning the repository.
git clone https://github.com/pixelgentechnologies/pixelatorR.git
Then, in an R session, run:
install.packages("<path to pixelatorR directory>", repos = NULL, type = "source")
Usage
Visit our tutorials for a step-by step guide on MPX/PNA data analysis with pixelatorR. On this site, we also provide additional additional tutorials that are more directed towards users who are interested in the details of how pixelatorR stores and handles MPX/PNA data in R.
Function documentation can be accessed from the reference tab above. Alternatively, you can access the documentation from an R session by running ?function_name
in the console once the package is installed.
Notes
pixelatorR is designed to work with classes from the SeuratObject R package. For the best analysis experience, we recommend installing Seurat v5.
Contact
For feature requests or bug reports, please use the GitHub issues.
You can also email the development team at developers@pixelgen.com.
Credits
pixelatorR is developed and maintained by the developers at Pixelgen Technologies.
When using pixelatorR in your research, please cite the following publication:
Karlsson, F., Kallas, T., Thiagarajan, D. et al. Molecular pixelation: spatial proteomics of single cells by sequencing. Nat Methods 21, 1044–1052 (2024). https://doi.org/10.1038/s41592-024-02268-9.
Package maintainer:
- Ludvig Larsson (@ludvigla)
Contributors:
- Max Karlsson (@maxkarlsson)
- Vincent Van Hoef (@vincent-van-hoef)
- Stefan Petkov (@stefanppetkov)
A huge thank you to all code contributors!